The localization of DCX-EMAP is achieved via a two-step process. (A) Representative confocal images showing the localization of NompC and DCX-EMAP in wild type and nompA^1/2. The blue and yellow arrowheads indicate the distal and proximal ends of the outer segment, respectively. The number of cells observed for each genotype is indicated. Scale bar, 5 µm. The cartoon schematics showing the localization of NompC and DCX-EMAP in the outer segment of wild type and nompA^1/2 are shown in the right panel. Genotypes: DCX-EMAP-gal4, uas-cd4-tdtom/uas-nompC-gfp. nompA¹/nompA²; DCX-EMAP-gal4, uas-cd4-tdtom/uas-nompC-gfp. DCX-EMAP-gal4, uas-cd4-tdtom/DCX-EMAP^KI. nompA¹/nompA²; DCX-EMAP-gal4, uas-cd4-tdtom/DCX-EMAP^KI. (B) Upper panel: Representative confocal images showing two types of localizations of GFP-DCX-EMAP in Klp64D^i-40945 (DCX-EMAP-gal4, uas-cd4-tdtom/Klp64D^i-40945; DCX-EMAP^KI). The white arrowhead in the images of the type II localization indicates a DCX-EMAP signal within the outer segment. The number of cells falling into each type of localization is indicated. Scale bar, 5 µm. Inset scale bar, 2 μm. Lower panel: Cartoon schematics showing the corresponding localization of GFP-DCX-EMAP in Klp64D^i-40945. The black arrowhead indicates the outer segment. (C) Upper panel: Representative confocal images showing the localization of DCX-EMAP in DSas-4^S2214 (DCX-EMAP-gal4, uas-cd4-tdtom/uas-gfp-DCX-EMAP; DSas-4^S2214). The white arrowhead indicates the distalmost tip of the leg receptor, where the dome-like cuticle structure was. Scale bar, 5 µm. Lower panel: Cartoon schematics showing the localization of DCX-EMAP in DSas-4^S2214. The two-sided arrow indicates the gap between the dendritic tip to the dome-like cuticle structure of the leg receptor.