New expression of CD3δ triggers RESET of PrP*. Time-lapse imaging of a representative N2a cell 48 h after transfection with YFP-PrP*, followed by a second round of transient transfection with CD3δ-CFP. Image collection was started 2 h after transfection with CD3δ-CFP. As with all time-lapse imaging, the same cell was imaged over time. Typical of N2a cells, this cell is mobile and rolls around several times over the course of this time-lapse sequence. Scale bar, 10 μm.