Deoxynojirimycin, but not thapsigargin or dithiothreitol, induces RESET of misfolded GPI-APs through a mechanism that requires constitutive protein translation. (A) Time-lapse imaging of representative N2a cell 48 h after transient transfection with YFP-PrP* and treated with 2.5 mM 1-deoxynojirimycin (DNJ) alone, or DNJ and 50 μg/ml cycloheximide (DNJ+CHX). (B) Time-lapse imaging of representative NRK cells that were stably expressing GFP-CD59 C94S after treatment with DNJ alone, or DNJ+CHX. (C) Time-lapse imaging of representative YFP-PrP* NRK cells after treatment with 1 μM thapsigargin alone (TG) or with TG+CHX, or 5 mM dithiothreitol alone (DTT) or with CHX (DTT+CHX). Scale bar, 10 μm.