Inhibiting ERAD increases the flux of disease mutant YFP-PrP M129V F198S through the RESET pathway. (A and B) Time-lapse imaging of representative NRK cells transiently transfected with prion disease mutant, YFP-PrP M129V F198S, and (A) Cerulean (CER)-CNX or (B) CER-GalT after treatment with 1 μM thapsigargin. (C) Time-lapse imaging of a representative NRK cell transiently transfected with YFP-PrP M129V F198S and CER-GalT after treatment with 10 μM NMS-873. Scale bar, 10 μm.