Role of CDC42 isoforms in dextran uptake and in non-chemotactic migration. (A) Localization of GFP-CDC42b or GFP-CDC42u in astrocytes pretreated overnight with an inhibitor of palmitoylation (2-bromopalmitate, 2BP, 120 µM) or an inhibitor of geranylgeranylation and/or palmitoylation (GGTI, 20 µM). (B) Histogram showing the relative dextran uptake observed in treated cells compared to control cells (Ctl). The graph shows the data and means ± SEM from three independent experiments. At least 150 cells per condition were analyzed. All P values were calculated using a two-sided unpaired Student’s t test. Scale bars: 10 µm. (C) Directional persistence (measured between 100 and 300 min after plating) of NPCs transfected with the indicated siRNA and migrating out of neurospheres. (D) Quantification of relative dextran uptake in NPCs transfected with the indicated siRNA. (E and F) Astrocyte (E) and NPC (F) migration in Boyden chamber-based xCelligence system assays. Graphs show the curve slopes indicative of the rate of migration in non-chemotactic conditions (no FBS in the lower well). Histograms show data points and means ± SEM of three to five independent experiments. At least 250 cells per condition were analyzed. All P values were calculated using two-sided unpaired Student’s t test. Scale bars: 10 µm.