Differential expression and localization of CDC42 isoforms in astrocytes. (A–D): (A) QPCR data using a TaqMan assay specifically detecting the ubiquitous CDC42u in astrocytes transfected with the indicated siRNA specific for each distinct isoform (si-u1, si-u2 and si-b1, si-b2) or targeting both isoforms (si-both). (B) Total CDC42 mRNA levels were measured using a TaqMan assay that recognizes both CDC42 isoforms (panCDC42) in astrocytes transfected with the indicated siRNA. (C) Graph showing the number of filopodia formation per mm length of wound edge post 1 h of scratch wound in astrocytes transfected with the indicated siRNA. (D) Confocal images of fixed astrocytes expressing ALFA-tagged CDC42 constructs 4–6 h after microinjection and stained with cis-Golgi marker GM130.