Related to Fig. 1 . mTurq2-Col4a1 basement membrane localization in adult epidermis and kidney. (A–F) Additional representative images from a second biological replicate mTurq2-Col4a1/+ adult mouse. (A and B) Dorsal skin sections from mTurq2-Col4a1/+ adult mouse labeled with ColIV (A and A’; magenta) or Perlecan (B and B’, magenta) and E-Cadherin (grayscale). mTurq2-COL4A1 (cyan) localizes to the basement membrane underlying the interfollicular epidermis and the dermal vasculature. Scale bars, 20 µm. (C) Additional biological replicate of kidney sections from mTurq2-Col4a1/+ adult mouse labeled with ColIV antibodies (magenta) and Hoechst to mark nuclei (blue). Scale bar, 100 µm. (D–D’’) High magnification view of mTurq2-COL4A1 (cyan) localization in the kidney of second biological replicate mTurq2-Col4a1/+ adult mouse labeled with ColIV (magenta in D; grayscale in D’) Hoechst (blue). Scale bar, 100 µm. (E) Kidney section from mTurq2-Col4a1/+ adult mouse labeled with Perlecan antibodies (magenta) and Hoechst (blue). Scale bar, 100 µm. (F–F’’) High magnification view of mTurq2-COL4A1 (cyan) localization in the kidney of mTurq2-Col4a1/+ adult mouse labeled with Perlecan (magenta in F; grayscale in F’) Hoechst (blue). Scale bar, 100 µm.