Kapβ1 depletion leads to CAS mis-localization. (A) Steady-state distribution of endoCAS and endoKapβ1 in control MDCK cells and transfected with either scramble siRNA-Cy5 or siRNA specific for Kapβ1 (siRNA1 and siRNA2). Scale bar, 5 μm. (B) Western blot showing the depletion of Kapβ1 by siRNA1 and siRNA2. (C) N/C ratios of endoKapβ1 and endoCAS under siRNA conditions. Only cells labeled with siRNA-Cy5 were analyzed. The dashed line indicates N/C = 1. Box plots denote the median, first, and third quartiles. The whiskers represent the minimum and maximum values. (D) Immunostaining of endoRan and endoCAS in control MDCK cells and transfected with either scramble siRNA-Cy5 or siRNA specific for the Kapβ1 (siRNA1 and siRNA2). Scale bar, 5 μm. (E) N/C ratios of endoRan obtained at each of these conditions. The dashed line indicates N/C = 1. Box plots denote the median, first, and third quartiles. The whiskers represent the minimum and maximum values. N = 3 or 4 in all experiments. The data were tested for normality using a built-in function of GraphPad Prism software. P values were obtained using unpaired two-tailed t test. Source data are available for this figure: SourceData F6.