Figure 5.
CAS nuclear retention requires RanBP1 and RanBP2. (A) Immunostaining of endoRan and RanBP1/2 in cells expressing mCherry-CAS after silencing RanBP1 or RanBP2. Scale bar, 5 μm. (B and C) Cytoplasmic-to-nuclear (C/N) ratios of (B) endoRanBP1 and (C) (endoRanBP2) after siRNA treatment. (D) Silencing RanBP1 or RanBP2 shifts the N/C ratio of mCherry-CAS toward one. (E) N/C ratio of endoRan does not show a significant change after depleting RanBP1 and RanBP2 by siRNA. Dashed line indicates N/C = 1. Box plots denote the median, first, and third quartiles. The whiskers represent the minimum and maximum values. Median values are indicated next to the box plots. N = 3. The data were tested for normality using a built-in function of GraphPad Prism software. P values were obtained using an unpaired two-tailed t test.

CAS nuclear retention requires RanBP1 and RanBP2. (A) Immunostaining of endoRan and RanBP1/2 in cells expressing mCherry-CAS after silencing RanBP1 or RanBP2. Scale bar, 5 μm. (B and C) Cytoplasmic-to-nuclear (C/N) ratios of (B) endoRanBP1 and (C) (endoRanBP2) after siRNA treatment. (D) Silencing RanBP1 or RanBP2 shifts the N/C ratio of mCherry-CAS toward one. (E) N/C ratio of endoRan does not show a significant change after depleting RanBP1 and RanBP2 by siRNA. Dashed line indicates N/C = 1. Box plots denote the median, first, and third quartiles. The whiskers represent the minimum and maximum values. Median values are indicated next to the box plots. N = 3. The data were tested for normality using a built-in function of GraphPad Prism software. P values were obtained using an unpaired two-tailed t test.

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