Figure 3. SARS-CoV-2 ORF6 sequesters... | Rockefeller University Press

Figure 3.

$SARS-CoV-2 ORF6 sequesters Kapα to the cytoplasm but does not affect CAS nuclear retention. (A) Immunostaining of endoCAS in cells co-expressing Kapα-mCherry with eGFP-ORF6, ORF6-eGFP, or ORF6-eGFP-NES2. Scale bar, 5 μm. (B) N/C ratio showing that the localization of each ORF6 construct is largely cytoplasmic. (C) N/C ratio showing that Kapα-mCherry has a larger cytoplasmic fraction when ORF6 is present. (D) N/C ratio showing that the nuclear retention of endoCAS is largely unaffected by the sequestration of Kapα-mCherry in the cytoplasm. Dashed line indicates N/C = 1. Box plots denote the median, first, and third quartiles. The whiskers represent the minimum and maximum values. Median values are indicated next to the box plots. N = 3. The data were tested for normality using a built-in function of GraphPad Prism software. P values were obtained using an unpaired two-tailed t test.$

SARS-CoV-2 ORF6 sequesters Kapα to the cytoplasm but does not affect CAS nuclear retention. (A) Immunostaining of endoCAS in cells co-expressing Kapα-mCherry with eGFP-ORF6, ORF6-eGFP, or ORF6-eGFP-NES₂. Scale bar, 5 μm. (B) N/C ratio showing that the localization of each ORF6 construct is largely cytoplasmic. (C) N/C ratio showing that Kapα-mCherry has a larger cytoplasmic fraction when ORF6 is present. (D) N/C ratio showing that the nuclear retention of endoCAS is largely unaffected by the sequestration of Kapα-mCherry in the cytoplasm. Dashed line indicates N/C = 1. Box plots denote the median, first, and third quartiles. The whiskers represent the minimum and maximum values. Median values are indicated next to the box plots. N = 3. The data were tested for normality using a built-in function of GraphPad Prism software. P values were obtained using an unpaired two-tailed t test.