Figure 5. RABI-8 affects the... | Rockefeller University Press

Figure 5.

$RABI-8 affects the oligomerization state of SMGL-1. (A) GFP::SMGL-1 precipitated MC::SMGL-1 in a representative Co-IP assay. Loss of RABI-8 increased the amount of precipitated MC::SMGL-1. Levels of MC::SMGL-1 are normalized to the corresponding GFP (set to 1 in wild-type animals). (B) The NT of SMGL-1 was pulled down by the full-length, NT, and CT of SMGL-1 in a representative protein pull-down assay. (C) Superose 6 increase SEC elution profile of SMGL-1 (black), RABI-8 (blue), and a mixture of SMGL-1 and RABI-8 (red). Right, from top to bottom: SDS-PAGE of the peak fractions from the SMGL-1–RABI-8, SMGL-1, and RABI-8 elution. Source data are available for this figure: SourceData F5.$

RABI-8 affects the oligomerization state of SMGL-1. (A) GFP::SMGL-1 precipitated MC::SMGL-1 in a representative Co-IP assay. Loss of RABI-8 increased the amount of precipitated MC::SMGL-1. Levels of MC::SMGL-1 are normalized to the corresponding GFP (set to 1 in wild-type animals). (B) The NT of SMGL-1 was pulled down by the full-length, NT, and CT of SMGL-1 in a representative protein pull-down assay. (C) Superose 6 increase SEC elution profile of SMGL-1 (black), RABI-8 (blue), and a mixture of SMGL-1 and RABI-8 (red). Right, from top to bottom: SDS-PAGE of the peak fractions from the SMGL-1–RABI-8, SMGL-1, and RABI-8 elution. Source data are available for this figure: SourceData F5.