Preferential impact of 2° and 3° LCs on the length of specific cell–cell contacts. A comparison of the effects of ablating the medioapical actomyosin network in 2° versus 3° LCs reveals distinct effects on LC–LC (2°-3°) and 2°-1° versus 3°-1° contact expansion. The analysis, conducted using two-way ANOVA with Sídák’s multicomparison, demonstrates that ablating the network in 2° LCs has a greater impact on 2°-3° contact expansion than when the network is ablated in 3° LCs (mean R-differences of 0.4388, P = 0.0338, N = 10 from four eyes). Conversely, ablating the network in 3° LCs has a greater effect on 3°-1° versus 2°-1° contact expansion compared with ablating the network in 2° LCs (mean R-differences of 0.5305; P = 0.0095, N = 10 from four eyes).