Medioapical F-actin network can reassemble after ablation. (A and A′) After (A) partial or (A′) complete ablation, medioapical F-actin recoils and over time it reassembles. Top: Snapshots of a lattice edge from a time-lapse movie. Bottom: A magnified view of the boxed 2° LC. The yellow star marks the ablated region. White arrowheads mark the LC–LC contact for this and subsequent images. (B) Medioapical F-actin in 1° LC also recovers after ablation. Time series montage of F-actin recovery in a 1° LC over a period of 19 min. Scale bar = 3 µm.