Phosphoproteomic data uncovers downstream substrates from other mitotic kinases. (A) Volcano plots [Log10 (P value) versus Log2 ratio] representing protein abundance that are affected by the loss of TBK1. Proteins are shown in black circles. The inset indicated additional color coding for the statistical analysis. (B) Violin plot of identified phosphorylation substrates grouped by predicted kinase family representing phosphorylation sites affected by the loss of TBK1. Proteins are shown in gray open circles. Wilcoxon signed rank test to “Other”: alpha = 0.01. * P < 0.5, ns = not significant. (C) Volcano plots [Log2 ratio versus Log2 ratio] for representing phosphorylation sites that are affected by MRT67307 in TBK1 KO cells to distinguish impacts solely due to pharmacological treatment or genotype. Proteins are shown in gray open circles. Circles were color coded for the motif that most likely fit TBK1 (blue), Aurora kinases (red), PAK kinases (yellow), Aurora or PAK kinases (orange), or all three (green). (D) Volcano plots [Log10 (P value) versus Log2 ratio] for representing phosphorylation sites that are affected byMRT67307 and loss of TBK1. Proteins are shown in black circles (non-significant) and red circle (Tier 1 significant). Circles were color coded for the motif that most likely fit TBK1 (blue), Aurora kinases (red), PAK kinases (yellow), Aurora or PAK kinases (orange), or all three (green). Bolded black circles categorize known mitotic proteins (GO:0000278). The inset indicated additional color coding for the statistical analysis.