Principal component analysis (PCA) of the phosphoproteome data and experimentally derived substrate sequence specificity for Aurora family, PAK famliy, TBK1, and IKKε kinases. (A) Principal component analysis (PCA) of the phosphoproteome data. Replicate samples are shown separately. 39.7% of the changes in phosphoabundance are provided by Component 1, which represents the genetic background component, while 27% of the change are provided by Component 2, which represents the small molecule inhibitortreatment. (B) Individual motifs for experimentally derived substrate sequence specificity for Aurora family, PAK family, TBK1 and IKKe kinases. Data derived from Johnson et al. (2023).