Misregulation of the FzlA-FtsK-FtsWI pathway results in DNA damage. (A–C) Percentage of cells with high levels of GFP from the P_sidA-gfp reporter. Plots comparing high GFP-producing cells between (A) WT (EG3653), ftsW**I* (EG3655), and ΔfzlA; ftsW**I* (EG3667) backgrounds or (B) strains containing either an EV control or fzlA-overexpression (OE) construct in WT (EV: EG3658, fzlA OE: EG3664) or ftsW**I* (EV: EG3666, fzlA OE: EG3672) backgrounds in the presence of 0.3% xylose for 4, 6, and 8 h. Averages of biological triplicates are shown and were used for statistical analysis. (C) Plot comparing high GFP-producing cells grown in 0.3% xylose for 4 h of a full-length FtsK_FL depletion strain in a WT background (EG3904 + xyl 4hr) to various depletion strains grown in the presence or absence of 0.3% xylose (EG3904: FtsK depletion in WT, EG3900: FtsK_C depletion in WT, EG3902: FtsK depletion in ftsW**I*) at 4, 6, and 8 h. Averages of biological triplicates are shown and were used for statistical analysis. *P < 0.05. **P < 0.01, ***P < 0.001, ****P < 0.0001.