Figure 5.
FzlA moves with active FtsWI. (A) Histogram comparing speeds of FzlA molecules in halo-fzlA; WT (EG3619, n = 104 total from three biological replicates) or halo-fzlA; ftsW**I* (EG3617, n = 71 total from four biological replicates) backgrounds. (B) Two-population fitting of FzlA single molecule movement data from A. (i and iii) Two-population fit to FzlA single molecule movement in WT (EG3619) (i) and ftsW**I* (EG3617) (iii). Orange and blue curves represent the slow- and fast-moving populations, respectively. The black dashed curve is the overall fit of the distribution. (ii and iv) Goodness of fit (top) of two- (magenta) versus one- (blue) population fitting of halo-fzlA speed distribution. The residuals of each fit (bottom) illustrate how well each model captures the data at every point along the CDF. (C) Plot comparing the move times of FzlA molecules moving at speeds <20 nm/s in halo-fzlA; WT (EG3619, n = 93 total from three biological replicates) or halo-fzlA; ftsW**I* (EG3617, n = 55 total from four biological replicates) backgrounds. Error bars are SEM. (D) Fraction of the FzlA population that is moving overall (% moving), slow moving, or fast moving for the indicated strains and conditions.

FzlA moves with active FtsWI. (A) Histogram comparing speeds of FzlA molecules in halo-fzlA; WT (EG3619, n = 104 total from three biological replicates) or halo-fzlA; ftsW**I* (EG3617, n = 71 total from four biological replicates) backgrounds. (B) Two-population fitting of FzlA single molecule movement data from A. (i and iii) Two-population fit to FzlA single molecule movement in WT (EG3619) (i) and ftsW**I* (EG3617) (iii). Orange and blue curves represent the slow- and fast-moving populations, respectively. The black dashed curve is the overall fit of the distribution. (ii and iv) Goodness of fit (top) of two- (magenta) versus one- (blue) population fitting of halo-fzlA speed distribution. The residuals of each fit (bottom) illustrate how well each model captures the data at every point along the CDF. (C) Plot comparing the move times of FzlA molecules moving at speeds <20 nm/s in halo-fzlA; WT (EG3619, n = 93 total from three biological replicates) or halo-fzlA; ftsW**I* (EG3617, n = 55 total from four biological replicates) backgrounds. Error bars are SEM. (D) Fraction of the FzlA population that is moving overall (% moving), slow moving, or fast moving for the indicated strains and conditions.

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