Figure S4.
FzlA converts FtsW into a slow-moving, active state. Two-population fitting of the histograms of FtsW-HaloTag or FtsW**-HaloTag molecules under different conditions. The orange and blue curves represent the slow- and fast-moving populations, respectively. The black dashed curve is the overall fitting profile of the distribution. (A i and iii) (i) FtsW in FzlA-producing cells (EG3523 + 0.001% xylose); (iii) FtsW in FzlA-depleted cells (EG3523 + 0.001% glucose). (B i, iii, v, and vii) (i) FtsW in EV control cells; (iii) FtsW in FzlA-overproducing cells; (v) FtsW** in ftsW**I* EV control cells; (vii) FtsW** in ftsW**I* FzlA-overproducing cells. (A ii and iv; and B ii, iv, vi, and viii) The fit goodness of one- (blue) and two- (magenta) population fitting of the corresponding conditions. The top sub-figures are the CDF curves and the fit curves, while the bottom figures show the residuals of the fitting.

FzlA converts FtsW into a slow-moving, active state. Two-population fitting of the histograms of FtsW-HaloTag or FtsW**-HaloTag molecules under different conditions. The orange and blue curves represent the slow- and fast-moving populations, respectively. The black dashed curve is the overall fitting profile of the distribution. (A i and iii) (i) FtsW in FzlA-producing cells (EG3523 + 0.001% xylose); (iii) FtsW in FzlA-depleted cells (EG3523 + 0.001% glucose). (B i, iii, v, and vii) (i) FtsW in EV control cells; (iii) FtsW in FzlA-overproducing cells; (v) FtsW** in ftsW**I* EV control cells; (vii) FtsW** in ftsW**I* FzlA-overproducing cells. (A ii and iv; and B ii, iv, vi, and viii) The fit goodness of one- (blue) and two- (magenta) population fitting of the corresponding conditions. The top sub-figures are the CDF curves and the fit curves, while the bottom figures show the residuals of the fitting.

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