Figure S2.
Phospho-null NuSAP resulted in delayed mitosis progression. (A) RFP-H2B (red) expressing HeLa cells with NuSAP siRNA knockdown were processed for rescue experiments with GFP-tagged NuSAP-WT, -S240A, and -S240D (green) followed by live-cell imaging. Images were captured every 3 min with five slices of 8-μm Z-section thickness. (B) Detection of NuSAP RNAi knockdown efficiency in HeLa cells by Western blotting. (C) Quantification of the time from NEBD to chromosome segregation, shown in A. Error bars indicate SD. Three independent replicates of 10 cells per replicate were quantified (n = 3). Unpaired two-tailed t test. P < 0.0001 for WT/S240A. P < 0.0001 for S240A/S240D. ****, P < 0.0001. n = the number of independent experiments presented. Scale bars, 10 μm. Source data are available for this figure: SourceData FS2.

Phospho-null NuSAP resulted in delayed mitosis progression. (A) RFP-H2B (red) expressing HeLa cells with NuSAP siRNA knockdown were processed for rescue experiments with GFP-tagged NuSAP-WT, -S240A, and -S240D (green) followed by live-cell imaging. Images were captured every 3 min with five slices of 8-μm Z-section thickness. (B) Detection of NuSAP RNAi knockdown efficiency in HeLa cells by Western blotting. (C) Quantification of the time from NEBD to chromosome segregation, shown in A. Error bars indicate SD. Three independent replicates of 10 cells per replicate were quantified (n = 3). Unpaired two-tailed t test. P < 0.0001 for WT/S240A. P < 0.0001 for S240A/S240D. ****, P < 0.0001. n = the number of independent experiments presented. Scale bars, 10 μm. Source data are available for this figure: SourceData FS2.

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