OSBP-dependent PI(4)P consumption in epithelial and mesenchymal cells and meta-analysis of OSBP-expression. (A and B) Results of three independent live imaging experiments using MDCK-PI4M-SidM (A) and P4M-SidM-expressing A549 cells (B). Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate 95% confidence interval. n = number of cells analyzed/experiment to obtain mean and 95% CI. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ^‡ indicates the representative experiments shown in Fig. 5, A and B, respectively. (C) Correlation matrix comparing ORP expression values and epithelial-mesenchymal index corresponding to cell lines included in the Cancer Cell Line Encyclopedia. Corr = Pearson r, *P < 0.05, **P < 0.01, ***P < 0.005. (D) Gene expression heatmap containing expression values for mesenchymal, epithelial marker genes as well as ORP genes of TCGA lung adenocarcinoma cohort. (E) Correlation matrix comparing ORP expression values and epithelial–mesenchymal indexes corresponding to tumor specimens included in the TCGA lung adenocarcinoma cohort. Corr = Pearson r, *P < 0.05, **P < 0.01, ***P < 0.005. (F) Calculated epithelial–mesenchymal indexes corresponding to each lung adenocarcinoma patient sample. (G) Correlations between epithelial-mesenchymal index and ORP expression values in TCGA lung adenocarcinoma samples. (H) Histology samples from The Human Protein Atlas show high epithelial-specific OSBP and OSBPL2 staining.