Figure S4.
D4H-EGFP distribution and lipid order imaging in MDCK cells upon OSBP silencing and inhibition. (A and B) Additional cysts to show the effect of OSBP silencing (A) or SWG-treatment (B) on D4H-GFP distribution. Bar = 10 μm. (C–E) Results of three independent experiments on the effect of SWG-treatment on MDCK plasma membrane lipid order. (C) Representative pseudo-colored micrographs of each independent experiment. (D) Histograms showing ratio values in DMSO and SWG-treated MDCK cells across the three repeats. (E) The mean values obtained from each independent experiment were compared by one-way ANOVA with Dunnett’s post hoc test. *P < 0.05. Histograms show the mean ± SEM of four fields, each containing at least 8–10 cells. Triangles point to mean values. Bar = 25 μm. ‡ indicates the representative experiment shown in Fig. 5 D. (F–H) Results of three independent experiments on the effect of OBP silencing on MDCK plasma membrane lipid order. (F) Representative pseudo-colored micrographs of each independent experiment. (G) Histograms showing ratio values in control and OSBP-silenced MDCK cells across the three repeats. (H) The mean values obtained from each independent experiment were compared by paired t test. *P < 0.05. Histograms show the mean ± SEM of four fields, each containing at least 8–10 cells. Triangles point to mean values. Bar = 25 μm. ‡ indicates the representative experiment shown in Fig. 5 E. (I–K) Results of three independent experiments on the effect SWG treatment on polarized MDCK plasma membrane lipid order. (I) Representative pseudo-colored micrographs of each independent experiment. (J) Histograms showing domain-specific ratio values in control and SWG-treated MDCK cells across the three repeats. (E) The mean values obtained from each independent experiment were compared by paired t test. *P < 0.05, **P < 0.01. Histograms show the mean ± SEM of four fields, each containing at least 8–10 cells. Triangles point to mean values. Bar = 10 μm. ‡ indicates the representative experiment shown in Fig. 5 F.

D4H-EGFP distribution and lipid order imaging in MDCK cells upon OSBP silencing and inhibition. (A and B) Additional cysts to show the effect of OSBP silencing (A) or SWG-treatment (B) on D4H-GFP distribution. Bar = 10 μm. (C–E) Results of three independent experiments on the effect of SWG-treatment on MDCK plasma membrane lipid order. (C) Representative pseudo-colored micrographs of each independent experiment. (D) Histograms showing ratio values in DMSO and SWG-treated MDCK cells across the three repeats. (E) The mean values obtained from each independent experiment were compared by one-way ANOVA with Dunnett’s post hoc test. *P < 0.05. Histograms show the mean ± SEM of four fields, each containing at least 8–10 cells. Triangles point to mean values. Bar = 25 μm. ‡ indicates the representative experiment shown in Fig. 5 D. (F–H) Results of three independent experiments on the effect of OBP silencing on MDCK plasma membrane lipid order. (F) Representative pseudo-colored micrographs of each independent experiment. (G) Histograms showing ratio values in control and OSBP-silenced MDCK cells across the three repeats. (H) The mean values obtained from each independent experiment were compared by paired t test. *P < 0.05. Histograms show the mean ± SEM of four fields, each containing at least 8–10 cells. Triangles point to mean values. Bar = 25 μm. ‡ indicates the representative experiment shown in Fig. 5 E. (I–K) Results of three independent experiments on the effect SWG treatment on polarized MDCK plasma membrane lipid order. (I) Representative pseudo-colored micrographs of each independent experiment. (J) Histograms showing domain-specific ratio values in control and SWG-treated MDCK cells across the three repeats. (E) The mean values obtained from each independent experiment were compared by paired t test. *P < 0.05, **P < 0.01. Histograms show the mean ± SEM of four fields, each containing at least 8–10 cells. Triangles point to mean values. Bar = 10 μm. ‡ indicates the representative experiment shown in Fig. 5 F.

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