Figure S2.
Effects of OSBP inhibition on apical and basolateral cargo trafficking and cyst morphology. (A–D) Results of three independent experiments using CDH1, DSG2, EGFP-GPI, and GP135 RUSH model cargoes co-transfected with mCherry-OSBPHHK>AAA upon OSW-1 treatment. Similar to Fig. 2, A–D, the fluorescence intensities obtained for the two detection channels (EGFP-cargoes, mCherry-OSBPHHK>AAA) are shown on separate plots. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. *P < 0.05. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in Fig. 2, A–D. (E) Additional images to show the effect of SWG treatment on MDCK cyst morphology. * indicates cyst shown in Fig. 2 E. Bar = 20 μm.

Effects of OSBP inhibition on apical and basolateral cargo trafficking and cyst morphology. (A–D) Results of three independent experiments using CDH1, DSG2, EGFP-GPI, and GP135 RUSH model cargoes co-transfected with mCherry-OSBPHHK>AAA upon OSW-1 treatment. Similar to Fig. 2, A–D, the fluorescence intensities obtained for the two detection channels (EGFP-cargoes, mCherry-OSBPHHK>AAA) are shown on separate plots. Thin lines indicate individual cells. Bold lines correspond to means, while shaded areas indicate SEM. n = number of cells analyzed/experiment to obtain mean and SEM. *P < 0.05. P values were calculated by two-way ANOVA followed by Sidak’s multiple comparison test. ‡ indicates the representative experiments shown in Fig. 2, A–D. (E) Additional images to show the effect of SWG treatment on MDCK cyst morphology. * indicates cyst shown in Fig. 2 E. Bar = 20 μm.

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