Screening behavior of microglia along myelin sheaths. (A) Microglia motility along myelin sheaths was monitored in 3-min acquisitions over 1 h by confocal time-lapse imaging of a Tg (mpeg1:EGFP; sox10:mRFP) zebrafish spinal cord. (B) XY plot correlates the mean number of microglia present along the spinal cord with the mean percentage of frames in which microglia remained static. Data points represent individual larvae. Linear regression analysis: R2: 0.1669, P = 0.4213. Dotted lines represent the standard error of the linear regression. (C) Heatmaps of microglial presence in quadrants of the dorsal and ventral spinal cord from five other fish (see Fig. 3 F for Fish 1). (D) Confocal time-lapse imaging of motile mbp:mCherry-CAAX fragments within a microglia in a 7 dpf Tg (mpeg1:EGFP; mbp:mCherry-CAAX) spinal cord. Z-stacks were acquired every 3 min. Images show maximum projections. Scale bars: 5 µm (D), 20 µm (A). See also Video 4.
