Figure S2.
Quality control for wild-type and tub1Δ/TUB1 cells. (A) Fraction of colonies recovered for each genotype used in tubulin quantification experiments. Dots are colored by experiment. Bars represent mean ± 95% CI. (B) Plot of estimated cells loaded vs. α-tubulin intensity for wild-type cells with a line of best fit. Data are colored by experiment and shape indicates biological replicate. (C) Plot of volume loaded vs. Zwf1 (G6PD) loading control intensity on the α-tubulin Western blot for wild-type cells with a line of best fit. Data are colored by experiment and shape indicates biological replicate. (D) Plot of estimated cells loaded vs. β-tubulin intensity for wild-type cells with a line of best fit. Data are colored by experiment and shape indicates biological replicate. (E) Plot of volume loaded vs. Zwf1 (G6PD) loading control intensity on the β-tubulin Western blot for wild-type cells with a line of best fit. Data are colored by experiment and shape indicates biological replicate. (F) Plot of purified tubulin loaded vs. α-tubulin intensity with a line of best fit. Data are colored by experiment. (G) Plot of purified tubulin loaded vs. β-tubulin intensity with a line of best fit. Data are colored by experiment. (H) Plot of estimated cells loaded vs. α-tubulin intensity for tub1Δ/TUB1 cells with a line of best fit. Data are colored by experiment and shape indicates replicate. (I) Plot of volume loaded vs. Zwf1 (G6PD) loading control intensity on the α-tubulin Western blot for tub1Δ/TUB1 cells with a line of best fit. Data are colored by experiment and shape indicates replicate. (J) Plot of estimated cells loaded vs. β-tubulin intensity for tub1Δ/TUB1 cells with a line of best fit. Data are colored by experiment and shape indicates replicate. (K) Plot of volume loaded vs. Zwf1 (G6PD) loading control intensity on the β-tubulin Western blot for tub1Δ/TUB1 cells with a line of best fit. Data are colored by experiment and shape indicates replicate. (L) Plot of purified tubulin loaded vs. α-tubulin intensity with a line of best fit. Data are colored by experiment. (M) Plot of purified tubulin loaded vs. β-tubulin intensity with a line of best fit. Data are colored by experiment.

Quality control for wild-type and tub1Δ/TUB1 cells. (A) Fraction of colonies recovered for each genotype used in tubulin quantification experiments. Dots are colored by experiment. Bars represent mean ± 95% CI. (B) Plot of estimated cells loaded vs. α-tubulin intensity for wild-type cells with a line of best fit. Data are colored by experiment and shape indicates biological replicate. (C) Plot of volume loaded vs. Zwf1 (G6PD) loading control intensity on the α-tubulin Western blot for wild-type cells with a line of best fit. Data are colored by experiment and shape indicates biological replicate. (D) Plot of estimated cells loaded vs. β-tubulin intensity for wild-type cells with a line of best fit. Data are colored by experiment and shape indicates biological replicate. (E) Plot of volume loaded vs. Zwf1 (G6PD) loading control intensity on the β-tubulin Western blot for wild-type cells with a line of best fit. Data are colored by experiment and shape indicates biological replicate. (F) Plot of purified tubulin loaded vs. α-tubulin intensity with a line of best fit. Data are colored by experiment. (G) Plot of purified tubulin loaded vs. β-tubulin intensity with a line of best fit. Data are colored by experiment. (H) Plot of estimated cells loaded vs. α-tubulin intensity for tub1Δ/TUB1 cells with a line of best fit. Data are colored by experiment and shape indicates replicate. (I) Plot of volume loaded vs. Zwf1 (G6PD) loading control intensity on the α-tubulin Western blot for tub1Δ/TUB1 cells with a line of best fit. Data are colored by experiment and shape indicates replicate. (J) Plot of estimated cells loaded vs. β-tubulin intensity for tub1Δ/TUB1 cells with a line of best fit. Data are colored by experiment and shape indicates replicate. (K) Plot of volume loaded vs. Zwf1 (G6PD) loading control intensity on the β-tubulin Western blot for tub1Δ/TUB1 cells with a line of best fit. Data are colored by experiment and shape indicates replicate. (L) Plot of purified tubulin loaded vs. α-tubulin intensity with a line of best fit. Data are colored by experiment. (M) Plot of purified tubulin loaded vs. β-tubulin intensity with a line of best fit. Data are colored by experiment.

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