Alterative lysis of wild-type cells. (A) Plot of paired ng of α- or β-tubulin per µg of total protein lysate loaded for a 6,000 xg clarifying spin alone or a 6,000 xg spin followed by a 100,000 xg clarifying spin of wild-type cells. Data represent three independent experiments, and each dot is mean ng tubulin/µg total protein lysate across the dilutions. Dots are colored by experiment. (B) Ratio of α-to β-tubulin for the two spins. Ratios were calculated from data in A. Bars are mean ±95% CI. (C) Plot of µg total protein lysate loaded vs. α-tubulin intensity for wild-type cells with a line of best fit. Data are colored by experiment, and shape indicates spin conditions. (D) Plot of µg total protein lysate loaded vs. β-tubulin intensity for wild-type cells with a line of best fit. Data are colored by experiment and shape indicates spin conditions. (E) Plot of purified tubulin loaded vs. α-tubulin intensity for wild-type cells with a line of best fit. Data are colored by experiment. (F) Plot of purified tubulin loaded vs. β-tubulin intensity for wild-type cells with a line of best fit. Data are colored by experiment.