Par-1 loss from the posterior following PFC ablation is slower in stage 10A oocytes. (A) Two-color time-lapse images of a control experiment on an egg chamber expressing GFP::Par-1 in the germline (cyan) and Jupiter::mCherry (magenta) before (−1 min) and after ablation of main body follicle cells (yellow dashed circle). (B) Zoom-in of the lateral side of the egg chamber shown in A. Top: merged channels, bottom: GFP::Par-1. No change in Par-1 localization is observed. (C) Average intensity profile of GFP::Par-1 signal around the ablated region before (blue) and after (orange) ablation of the main body follicle cells. The shaded region designates the SD. The x-axis origin represents the center of the ablated region. (D) Two-color time-lapse images of an egg chamber at stage 10A expressing GFP::Par-1 (cyan) and Baz::mCherry (yellow) in the germline, before (−1 min) and after ablation of PFCs (red dashed circle). Scale bar, 20 µm. (E) Zoom into the posterior of the egg chamber shown in A. Top: GFP::Par-1, bottom: Baz::mCherry. Following ablation, Bazooka accumulates to the posterior, while Par-1 marginally delocalizes (arrowheads). Scale bar, 20 µm. (F) Average intensity profile of GFP::Par-1 (cyan) and Baz::mCherry (yellow) signal at the posterior of stage 10A oocytes before (top graph) and 1 h after ablation of PFCs (bottom graph). The shaded region designates the SD. The x-axis origin represents the position of the polar cells (top) or center of ablation spot (bottom). Note that Par-1 signal does not decrease much.