Figure S2.
Bazooka localizes to the posterior following ablation of PFCs. (A) Two-color time-lapse images of an egg chamber expressing Baz::GFP in the germline (yellow) and the microtubule marker Jupiter::mCherry (magenta) before (−1 min) and after ablation of PFCs. (B) Zoom-in of the posterior of the egg chamber shown in panel A. Top: merged channels, bottom: Baz::GFP. Dashed circle in the first image of the top panel represents the ablation spot. Note that polar cells (pair of cells with high Jupiter signal) are not ablated. After 80 min, the accumulation of Baz::GFP is visible at the oocyte membrane facing the ablated cells (arrowheads in last image). (C) Average intensity profile of Baz::GFP signal at the posterior of the oocyte before (blue) and after (orange) ablation. The shaded region represents the SD. The x-axis origin represents the position of the ablated region. Vertical lines denote the average width of the ablated region. Note that the signal increases markedly only within the ablated region. (D) Two-color time-lapse images of an egg chamber expressing Baz::GFP in the germline (yellow) and microtubule marker Jupiter::mCherry (magenta) before (−1 min) and after ablation within the ooplasm. (E) Zoom-in of the posterior of the egg chamber shown in panel D. Top: merged channels, bottom: Baz::GFP. Dashed circle in the first image of the bottom panel shows the position of the ablation spot. Bazooka remains excluded from the posterior after ablation (arrowheads). (F) Average intensity profile of Baz::GFP signal at the posterior of the oocyte before (blue) and after (orange) ablation within the ooplasm. The shaded region designates the SD. The x-axis origin represents the position of polar cells. For all panels the scale bar represents 20 μm, and n is the number of experiments.

Bazooka localizes to the posterior following ablation of PFCs. (A) Two-color time-lapse images of an egg chamber expressing Baz::GFP in the germline (yellow) and the microtubule marker Jupiter::mCherry (magenta) before (−1 min) and after ablation of PFCs. (B) Zoom-in of the posterior of the egg chamber shown in panel A. Top: merged channels, bottom: Baz::GFP. Dashed circle in the first image of the top panel represents the ablation spot. Note that polar cells (pair of cells with high Jupiter signal) are not ablated. After 80 min, the accumulation of Baz::GFP is visible at the oocyte membrane facing the ablated cells (arrowheads in last image). (C) Average intensity profile of Baz::GFP signal at the posterior of the oocyte before (blue) and after (orange) ablation. The shaded region represents the SD. The x-axis origin represents the position of the ablated region. Vertical lines denote the average width of the ablated region. Note that the signal increases markedly only within the ablated region. (D) Two-color time-lapse images of an egg chamber expressing Baz::GFP in the germline (yellow) and microtubule marker Jupiter::mCherry (magenta) before (−1 min) and after ablation within the ooplasm. (E) Zoom-in of the posterior of the egg chamber shown in panel D. Top: merged channels, bottom: Baz::GFP. Dashed circle in the first image of the bottom panel shows the position of the ablation spot. Bazooka remains excluded from the posterior after ablation (arrowheads). (F) Average intensity profile of Baz::GFP signal at the posterior of the oocyte before (blue) and after (orange) ablation within the ooplasm. The shaded region designates the SD. The x-axis origin represents the position of polar cells. For all panels the scale bar represents 20 μm, and n is the number of experiments.

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