Localization of Bazooka and Par-1 during stages 10 and 11 of oogenesis. (A and B) On the left are still images of stage 10A (A) and 11 (B) egg chambers expressing Baz::GFP (yellow) and the microtubule reporter Jupiter::mCherry (magenta) next to a transmission light (TL) micrograph (gray). The right graphs are intensity profiles of Baz::GFP (yellow), Jupiter::mCherry (magenta), and transmission light (gray) signal along a straight line crossing cell boundaries from the oocyte to either lateral or posterior follicle cells. The x-axis origin and the vertical line represent the local minimum of the Jupiter::mCherry (magenta) signal, which we interpret as extended intercellular space (gap) between the oocyte and the follicle cells. If the intercellular space is not clearly discernible, the position 0 μm represents the midpoint of the line. (A) At stage 10A, the gap is visible between the oocyte and the lateral follicle cells, but not between the oocyte and the posterior follicle cells. Accumulation of Bazooka correlates with the existence of the gap. (B) At stage 11, both the gap and Bazooka accumulation are visible at the posterior. (C–E) Stage 10A, 10B, and 11 egg chambers expressing Baz::mCherry (yellow) and GFP::Par-1 (cyan) in the germline. At stage 10A, the mutual exclusion zone between Bazooka and Par-1 exists at the posterior. During stage 10B Bazooka accumulates to the posterior before delocalization of Par-1. At stage 11, both exclusion of Bazooka and accumulation of Par-1 at the posterior are lost. (F) Distribution of egg chambers showing different posterior Par protein localization patterns. The colors in the plot correspond to the color of the frame surrounding the images in C–E. Note that Par-1 delocalization never precedes accumulation of Bazooka. Scale bars represent 20 μm, n is the number of egg chambers.