Figure 1. Bazooka accumulates at the... | Rockefeller University Press

Figure 1.

$Bazooka accumulates at the posterior following the loss of contact between the oocyte and PFCs at stage 10B of oogenesis. (A–C) On the left are still images of stage 10B egg chambers expressing Baz::GFP (yellow) and the microtubule reporter Jupiter::mCherry (magenta) next to a transmission light (TL) micrograph (gray). The right graphs are intensity profiles of Baz::GFP (yellow), Jupiter::mCherry (magenta), and transmission light (gray) signal along a straight line crossing cell boundaries from the oocyte to either lateral or posterior follicle cells (see first image of A). The x-axis origin and vertical line represent the local minimum of the Jupiter::mCherry (magenta) signal, which we interpret as extended intercellular space (gap) between the oocyte and the follicle cells. Whenever this intercellular space is not clearly discernible, the position 0 μm represents the midpoint of the line. (A) At the beginning of stage 10B, a gap is detected between the oocyte and the lateral follicle cells but not the posterior follicle cells. Accumulation of Bazooka at the oocyte membrane correlates with existence of the gap. (B) Later in stage 10B, a gap is formed at the posterior, but Bazooka does not yet accumulate at the respective oocyte membrane. (C) Eventually, Bazooka accumulates to the posterior after the formation of the gap. The scale bars represent 20 μm for A–C. (D) Distribution of egg chambers showing one of the four possible phenotypes at stages 9, 10A, 10B, and 11. Stage 9 is distinct from later stages in that a fraction of egg chambers shows no (anterolateral) gap at all. The colors in the plot correspond to the color of the frame surrounding the images in A–C. n is the number of egg chambers quantified. (E) Scheme showing the timeline of events during stage 10B. Loss of contact between the oocyte and PFCs precedes accumulation of Bazooka to the posterior.$

Bazooka accumulates at the posterior following the loss of contact between the oocyte and PFCs at stage 10B of oogenesis. (A–C) On the left are still images of stage 10B egg chambers expressing Baz::GFP (yellow) and the microtubule reporter Jupiter::mCherry (magenta) next to a transmission light (TL) micrograph (gray). The right graphs are intensity profiles of Baz::GFP (yellow), Jupiter::mCherry (magenta), and transmission light (gray) signal along a straight line crossing cell boundaries from the oocyte to either lateral or posterior follicle cells (see first image of A). The x-axis origin and vertical line represent the local minimum of the Jupiter::mCherry (magenta) signal, which we interpret as extended intercellular space (gap) between the oocyte and the follicle cells. Whenever this intercellular space is not clearly discernible, the position 0 μm represents the midpoint of the line. (A) At the beginning of stage 10B, a gap is detected between the oocyte and the lateral follicle cells but not the posterior follicle cells. Accumulation of Bazooka at the oocyte membrane correlates with existence of the gap. (B) Later in stage 10B, a gap is formed at the posterior, but Bazooka does not yet accumulate at the respective oocyte membrane. (C) Eventually, Bazooka accumulates to the posterior after the formation of the gap. The scale bars represent 20 μm for A–C. (D) Distribution of egg chambers showing one of the four possible phenotypes at stages 9, 10A, 10B, and 11. Stage 9 is distinct from later stages in that a fraction of egg chambers shows no (anterolateral) gap at all. The colors in the plot correspond to the color of the frame surrounding the images in A–C. n is the number of egg chambers quantified. (E) Scheme showing the timeline of events during stage 10B. Loss of contact between the oocyte and PFCs precedes accumulation of Bazooka to the posterior.