The optimized activation of PARP1 regulated mice anxiety and epileptic behavior. (A–H) The results of the PTZ kindling test. The chart representing the seizure stage classification of the scores from mice with indicated genotypes and treatments, before and after the PTZ injection (A and E). Statistical analysis results of the cumulative number of maximum (B and F) and all (C and G) seizure stage, and the ratio of the occurrence of stage 4 (D and H). Data are presented as mean ± SEM (n = 10 mouse pairs). **P < 0.01, ****P < 0.0001 (Student’s t tests). (I–M) The heatmap of the occupancy of individual mice with indicated pharmacological treatments in OFT. A warmer color represents more time spent (red > yellow > green > blue), and red rectangles are put at the central zones in the fields (I). The OFT results of individual mice with the indicated treatments. Total distances that mice ran during tests (J), cumulative durations in the edge zone (K) or the central zone (L), and in-zone frequencies to the central zone (M) are represented. Data are presented as mean ± SEM (n = 10 mouse pairs). n.s., P > 0.5, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (two-way ANOVA).