Binding strength between KIF4 and PARP1 was stronger in mutant KIF4. (A) Immunoblotting results of BioID. BirA indicated the mock vector transfected cells. Input or pull-down lysates were blotted with the indicated antibodies. Total biotinylated proteins were confirmed by streptavidin-conjugated horse radish peroxidase (Strep-HRP). Red arrows indicate the overexpressed KIF4 band and the black arrow indicates endogenous PARP1. (B) Immunocytochemistry of primary neurons using the indicated antibodies, mouse (MIgG), and rabbit IgG (RIgG); Scale bar, 10 μm. Source data are available for this figure: SourceData FS3.