Localization of ARHGAP17 domain deletion and CD mutants. (A) Representative TIRF micrographs of SUM159 cells expressing ARHGAP17-GFP domain deletion mutants in an ARHGAP17 KO background. Cells were treated with PDBu for 30 min and stained for F-actin as a marker for invadopodia. Arrowheads highlight invadopodia clusters (scale bar for both panels, 10 μm). (B) Representative TIRF micrographs of SUM159 cells expressing WT-ARHGAP17-GFP or CD-ARHGAP17-GFP in an ARHGAP17 KO background. Cells were treated with PDBu for 30 min and stained for F-actin as a marker for invadopodia. Arrowheads highlight invadopodia clusters. The zoomed regions are marked by a dashed line (scale bar for both panels, 10 μm).