Figure S4.
ARHGAP17-GFP localizes to invadopodia in non-treated SUM159 cells and MDA-MB-231 cells. (A) Representative micrographs of a SUM159 cell invadopodia cluster expressing TKS5-mTagBFP2 and ARHGAP17-GFP in an ARHGAP17 KO background (scale bar, 1 μm; inset scale bar, 0.5 μm). (B) Radial intensity analysis of TKS5-mTagBFP2 and ARHGAP17-GFP in SUM159 invadopodia. Lines are expressed as mean ± SEM of three independent experiments in which at least 10 invadopodia per condition per experiment were measured. (C) Representative micrographs of an MDA-MB-231 cell invadopodia cluster expressing TKS5-mScarlet and ARHGAP17-GFP in an ARHGAP17 KD background (scale bar, 1 μm; inset scale bar, 0.5 μm). (D) Radial intensity analysis of TKS5-mScarlet and ARHGAP17-GFP at MDA-MB-231 invadopodia. Lines are expressed as mean ± SEM of three independent experiments in which at least 10 invadopodia per condition per experiment were measured. (E) Radial intensity profiles of individual MDA-MB-231 invadopodia from live cells expressing TKS5-mScarlet and ARHGAP17-GFP (expressed in ARHGAP17 KD cells) plotted over time. Time scales between invadopodia are rescaled between 0 and 1 to allow for alignment of intensity values. Results are expressed as the mean of six representative invadopodia.

ARHGAP17-GFP localizes to invadopodia in non-treated SUM159 cells and MDA-MB-231 cells. (A) Representative micrographs of a SUM159 cell invadopodia cluster expressing TKS5-mTagBFP2 and ARHGAP17-GFP in an ARHGAP17 KO background (scale bar, 1 μm; inset scale bar, 0.5 μm). (B) Radial intensity analysis of TKS5-mTagBFP2 and ARHGAP17-GFP in SUM159 invadopodia. Lines are expressed as mean ± SEM of three independent experiments in which at least 10 invadopodia per condition per experiment were measured. (C) Representative micrographs of an MDA-MB-231 cell invadopodia cluster expressing TKS5-mScarlet and ARHGAP17-GFP in an ARHGAP17 KD background (scale bar, 1 μm; inset scale bar, 0.5 μm). (D) Radial intensity analysis of TKS5-mScarlet and ARHGAP17-GFP at MDA-MB-231 invadopodia. Lines are expressed as mean ± SEM of three independent experiments in which at least 10 invadopodia per condition per experiment were measured. (E) Radial intensity profiles of individual MDA-MB-231 invadopodia from live cells expressing TKS5-mScarlet and ARHGAP17-GFP (expressed in ARHGAP17 KD cells) plotted over time. Time scales between invadopodia are rescaled between 0 and 1 to allow for alignment of intensity values. Results are expressed as the mean of six representative invadopodia.

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