ARHGAP17 regulates invadopodia turnover, lifetime, and stability. (A) Quantification of change in percentage of cells forming invadopodia between peak invadopodia formation 10 min after PDBu treatment and equilibrium at 30 min after PDBu treatment. Results are expressed as mean ± SEM of three independent experiments in which at least 200 cells per condition per experiment were counted. Significance was tested with a two-tailed t test: *, P < 0.05; **, P < 0.01. (B and C) Quantification of invadopodia assembly and disassembly time (min). Results are expressed as mean ± SEM of three independent experiments in which at least 12 invadopodia per condition per experiment were measured. (D) Quantification of invadopodia lifetime in non-treated SUM159 cells. Results are expressed as mean ± SEM of at least 40 invadopodia per condition. Significance was tested with a two-tailed t test: ***, P < 0.001. (E and F) Quantification of invadopodia assembly and disassembly rate. Results are expressed as mean ± SEM of three independent experiments in which at least 12 invadopodia per condition per experiment were measured. In B–F, large circles represent biological replicates, small circles represent technical replicates. Significance was tested with a two-tailed t test. (G) Principal component analysis of invadopodia dynamics. The ellipses represent 95% confidence.