Identification of clathrin-coated pits, coated vesicles and caveolae. A coated pit model was generated by training with ground truth annotations from Cell 12 (Hela-2) prepared by HPFS and imaged at ∼5 nm isotropic resolution. (A) Orthogonal views of a representative endocytic clathrin-coated pit (CCP) not used for training showing ground truth annotations and model prediction. Scale bar, 50 nm. (B) Orthogonal views of a caveola, an endocytic clathrin-coated pit (CCP) and a clathrin-coated vesicle (CCV) at the plasma membrane, and a coated pit (CCP) and vesicle (CCV) associated with membranes from the secretory pathway. Each panel shows the ground truth annotation and the model prediction. An example of a COPI vesicle not predicted by the coated pit model is also shown. Views are from naïve cell 17 SVGA prepared by HPFS and imaged with ∼5 nm isotropic resolution.