Network predictions of mitochondria, ER, and Golgi apparatus. Single plane view from a FIB-SEM volume image from naïve cell 15 (SVG-A) not used for training prepared by HPFS and visualized during interphase at 5 nm isotropic resolution. The small region contains representative model predictions for mitochondria (cyan), ER (yellow), and Golgi apparatus (magenta) obtained from three 3D U-net models, each trained with organelle-specific ground truth annotations, without fine-tuning, from interphase cells 19 (Hela-2) and 20 (Hela-3) prepared by HPFS. Scale bar, 2 μm.