Figure 3.
Quantification of fusion activity using live imaging. (A) Time-lapse images from a fusion assay. BHK cells were transfected with plasmids for expression of RFPcyto (magenta) and EFF-1 (co-transfection) or IZUMO1-Venus (green). Arrowheads and arrows indicate contacting and fused cells, respectively. Time (h:min) after the start of observation (see Videos 2, 3, 4, 5, and 6). Sister cells generally fuse several hours after they divide, suggesting that the probability of fusion increases when the cells are at the same stage of the cell cycle. Scale bars, 50 µm. (B) Quantification of live imaging experiments in which BHK cells express RFPcyto, E-cadherin, EFF-1, GEX2, IZUMO1-Venus, or JUNO. The percentage of fusion was defined as the ratio between the number of fusion events (Fe) and the number of transfected cells (Tc), as follows: % of fusion events = (Fe/Tc) × 100. Bar chart showing individual experiments and means ± SEM of at least three independent experiments. Total Tc analyzed = 1,001 (RFP), 1,179 (E-cadherin), 930 (EFF-1), 415 (GEX2), 817 (IZUMO1), and 1,265 (JUNO). Comparisons by one-way ANOVA followed by Dunett’s test against RFPcyto. **P < 0.01. (C) Scheme of experimental design.

Quantification of fusion activity using live imaging. (A) Time-lapse images from a fusion assay. BHK cells were transfected with plasmids for expression of RFPcyto (magenta) and EFF-1 (co-transfection) or IZUMO1-Venus (green). Arrowheads and arrows indicate contacting and fused cells, respectively. Time (h:min) after the start of observation (see Videos 2, 3, 4, 5, and 6). Sister cells generally fuse several hours after they divide, suggesting that the probability of fusion increases when the cells are at the same stage of the cell cycle. Scale bars, 50 µm. (B) Quantification of live imaging experiments in which BHK cells express RFPcyto, E-cadherin, EFF-1, GEX2, IZUMO1-Venus, or JUNO. The percentage of fusion was defined as the ratio between the number of fusion events (Fe) and the number of transfected cells (Tc), as follows: % of fusion events = (Fe/Tc) × 100. Bar chart showing individual experiments and means ± SEM of at least three independent experiments. Total Tc analyzed = 1,001 (RFP), 1,179 (E-cadherin), 930 (EFF-1), 415 (GEX2), 817 (IZUMO1), and 1,265 (JUNO). Comparisons by one-way ANOVA followed by Dunett’s test against RFPcyto. **P < 0.01. (C) Scheme of experimental design.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal