Figure S3. Depleting fetal calf serum... | Rockefeller University Press

Figure S3.

$Depleting fetal calf serum (FBS) from FN with gelatin-sepharose. (A) Initial FBS, flow through (FN-depleted FBS), washes, and elution fractions were subjected to SDS-PAGE followed by immunoblot with P1H11 anti-FN antibody to verify FN removal. (B) Equal volumes of initial FBS and FN-depleted FBS were separated on SDS-PAGE and stained with Coomassie.$

Depleting fetal calf serum (FBS) from FN with gelatin-sepharose. (A) Initial FBS, flow through (FN-depleted FBS), washes, and elution fractions were subjected to SDS-PAGE followed by immunoblot with P1H11 anti-FN antibody to verify FN removal. (B) Equal volumes of initial FBS and FN-depleted FBS were separated on SDS-PAGE and stained with Coomassie.