Tensin- and paxillin-rich adhesions are involved in FN fibrillogenesis. Gallery of frames from a TIRFM video of a KO67 FN-Halo untagged β1itg fibroblast transiently transfected with a GFP-Tensin expression construct (paxillin-mCherry in red, GFP-tensin in green, and FN-Halo labeled with SiR-Halo ligand in blue) cultured on FN-coated glass. A zoomed-in view of the boxed area is shown below for each time point and separate channel pictures of the 3-h time point are shown in the lowest panel. Arrowheads point to growing fibrils of FN-Halo connected to tensin- and paxillin-positive adhesions. Scale bar = 10 μm. Source data are available for this figure: SourceData FS1.