Relative subcellular localization of RHGF-1 and NMY-1. (A) Maximum intensity projection of live super-resolution confocal images of RHGF-1::tagRFP and GFP::NMY-1 in an occupied spermatheca. Region in yellow box is enlarged on right. Scale bars: whole spermatheca, 10 µm; enlargement, 2 µm. (B) X-Z view at the surface of the spermatheca. Scale bar, 2 µm. (C) Intensity line profile based on 5-pixel line along X-Z view in B. (D) Maximum intensity projection made from a live super-resolution Z-stack of an occupied spermatheca in a worm expressing endogenous RHGF-1::tagRFP and GFP::NMY-1 under a spermatheca-specific promoter, following fln-1(RNAi). Region in yellow box is enlarged on right. Scale bars: whole spermatheca, 10 µm; enlargement, 2 µm.