Analysis of expression level and dwell time in all RHGF-1 deletion constructs. (A) A comparison of the fluorescence intensity of individual spermathecae in rhgf-1(ok880) worms expressing full-length or various deletion constructs of RHGF-1 fused to mKate. N ≥ 7. Bars are mean ± SEM. Statistical comparisons were performed by One-way ANOVA, P = 0.86 (non-significant). (B) A plot of dwell time as a function of the fluorescence intensity of the RHGF-1 construct expressed. Data includes all the constructs shown in A. Line is simple linear regression. A non-significant negative correlation (r = −0.05) was calculated by correlation assay. N = 43. N, number of samples analyzed.