The PDZ domain of RHGF-1 is required and sufficient for its basal recruitment. (A) Schematic presentation of RHGF-1 domains. (B–G) Confocal images showing the subcellular localization of mKate-fused full length RHGF-1 (B), PH domain deletion (C), RhoGEF domain deletion (D), C1 domain deletion (E), RGS domain deletion (F), and PDZ domain deletion (G), during spermatheca contraction. (H and I) Confocal images showing the subcellular localization of mKate-fused PDZ domain, when oocyte entry was completed and during embryo exit, respectively. Region in yellow box enlarged on right. Graphs show the fluorescence intensity profile along the yellow line from the apical side of the cell (“A”) and the basal side (“B”). Arrows point at cytoplasmic peaks and arrowheads point basal side peaks. Scale bars: whole spermatheca, 20 µm; enlargement, 2 µm. Full movies are in Videos 9 and 10.