Dwell time analyses in loss-of-function alleles and RNAi show that the RhoGEF unc-73 regulates spermatheca contractility and Y105E8A.25 does not. (A) Dwell time of wild-type, unc-73(ok322), unc-73(e936), unc-73(ce362), unc-73(ok936), Y105E8A.25(knu182), and Y105E8A.25(knu185) worms. N ≥ 3. Bars are mean ± SEM. Statistical comparisons were performed by One-way ANOVA-Tukey’s multiple comparisons test. (B) Dwell time in spv-1(ok1498) worms treated with spermatheca-specific unc-73(RNAi). N ≥ 7. Bars are mean ± SEM. Statistical comparison was performed by two-tailed unpaired t test. Stars designate statistical significance (*** P <0.001, ** P <0.01). N, number of samples analyzed.