Figure S2.
Lamp1 and endosomal Rab-GTPases associate with the trans-Golgi. (A) Confocal images showing localization of lysosome marker Lamp1-YFP (green) and trans-Golgi marker GalT-TagRFP (magenta, driven by act-GAL4) in L3 CNS, wing imaginal disc, and blood cells. Magnified insets are shown in the lower right corner of each image. Separate channels of magnified insets are shown on the right side. Nuclei stained with DAPI (blue). The graphs below each image quantify lysosome Lamp1 and trans-Golgi GalT puncta proximity measured in 10 different square areas (12.454 × 12.454 μm, CNS and wing disc) or 10 blood cells, as well as average proximity values (n = 10, error bars indicate SD). (B) Confocal images showing localization of Lamp1-YFP (green) and GalT-TagRFP (magenta, driven by act-GAL4) in L3 dorsal tracheal branch, salivary gland, midgut enteroblasts, and Malpighian tubules. Magnified insets are shown in the lower right corner of each image. Separate channels of magnified insets are shown on the right side. The graphs below each image quantify puncta proximity in 10 different cells, as well as average proximity values (n = 10, error bars indicate SD). (C) Confocal images of fat body (feeding stage) showing localization of trans-Golgi marker GalT-TagRFP (magenta) and fluorescently tagged Rab-GTPases Rab4, Rab5, Rab7, and Rab11 (green), all driven by BM-40-SPARC-GAL4. Magnified insets are shown in the lower right corner of each image, with separate channels on the right. The graphs below each image quantify Rab-GTPase and trans-Golgi GalT puncta proximity measured in 10 different cells, as well as average proximity values (n = 10, error bars indicate SD). Notice similar percentages of proximity compared to Fig. 2 L.

Lamp1 and endosomal Rab-GTPases associate with the trans-Golgi. (A) Confocal images showing localization of lysosome marker Lamp1-YFP (green) and trans-Golgi marker GalT-TagRFP (magenta, driven by act-GAL4) in L3 CNS, wing imaginal disc, and blood cells. Magnified insets are shown in the lower right corner of each image. Separate channels of magnified insets are shown on the right side. Nuclei stained with DAPI (blue). The graphs below each image quantify lysosome Lamp1 and trans-Golgi GalT puncta proximity measured in 10 different square areas (12.454 × 12.454 μm, CNS and wing disc) or 10 blood cells, as well as average proximity values (n = 10, error bars indicate SD). (B) Confocal images showing localization of Lamp1-YFP (green) and GalT-TagRFP (magenta, driven by act-GAL4) in L3 dorsal tracheal branch, salivary gland, midgut enteroblasts, and Malpighian tubules. Magnified insets are shown in the lower right corner of each image. Separate channels of magnified insets are shown on the right side. The graphs below each image quantify puncta proximity in 10 different cells, as well as average proximity values (n = 10, error bars indicate SD). (C) Confocal images of fat body (feeding stage) showing localization of trans-Golgi marker GalT-TagRFP (magenta) and fluorescently tagged Rab-GTPases Rab4, Rab5, Rab7, and Rab11 (green), all driven by BM-40-SPARC-GAL4. Magnified insets are shown in the lower right corner of each image, with separate channels on the right. The graphs below each image quantify Rab-GTPase and trans-Golgi GalT puncta proximity measured in 10 different cells, as well as average proximity values (n = 10, error bars indicate SD). Notice similar percentages of proximity compared to Fig. 2 L.

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