SRFBP1 is involved in Pol I regulation. (A) SIM imaging showing the co-localization of SRFBP1 with nucleolar markers in HeLa cells. FC: RPA43, DFC: FBL, GC: NPM1. Scale bar, 2 μm. Insert magnification: 2.7×. (B) Line scan of the relative fluorescence of the signal indicated by the dotted lines in A. (C) Relative position of the ChIP primers on the rDNA locus. (D and E) Bar plots showing ChIP-qPCR analysis of the relative enrichment of SRFBP1 (D), H3ac, or H3K14ac (E) at rDNA loci in control (NC) and SRFBP1 depleted Hela cells. Data are display as mean ± SEM. n = three technical repeats. Multiple t test, **P ≤ 0.01, *** P ≤ 0.001.