Figure 1.
Principle of LiveArt. Schematic illustration of LiveArt. 17 repeats of MS2 sequence were knocked into the rDNA transcribed region by CRISPR-Cas9 mediated HITI. Newly synthesized rRNA harboring MS2 hairpins are bound by stdMCP-tdTomato, enabling direct imaging of nascent rRNAs in living cells.

Principle of LiveArt. Schematic illustration of LiveArt. 17 repeats of MS2 sequence were knocked into the rDNA transcribed region by CRISPR-Cas9 mediated HITI. Newly synthesized rRNA harboring MS2 hairpins are bound by stdMCP-tdTomato, enabling direct imaging of nascent rRNAs in living cells.

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