Characterization of ADAMTS9/20 at the B-LINK. (A) Schematic timeline showing the timing of imaging. (B) Top: Fluorescence images of endogenous ADAMTS9/20::mNG (GON-1) at the B-LINK (white arrowheads) from mid L4 to peak egg-laying in the adult (n = 10 each). Bottom: Quantification of mean florescence intensity of ADAMTS9/20::mNG at each timepoint as a percentage of the final mean fluorescence intensity in the peak egg-laying adult. Error bars represent SEM (n = 10 all stages). Scale bar, 10 µm. (C) Mean fluorescence intensity of B-LINK matrix components α1-type IV collagen::mRuby2, mNG::fibulin-1, hemicentin::mNG, perlecan::mNG, and ADAMTS9/20::mNG on control (L4440/T444T empty vectors) and corresponding B-LINK matrix component RNAi. lin-35(n745) was used for fibulin-1 RNAi to sensitize worms for more efficient knockdown. Box edges in boxplots depict the 25th and 75th percentiles, the line in the box indicates the median value, and whiskers mark the minimum and maximum values. ****P < 0.0001, *P < 0.05, one-way ANOVA followed by post hoc Dunnet’s test, unpaired two-tailed t test for fibulin-1 RNAi.