Figure 9.
Hemicentin and fibulin-1 decline at the utse–seam B-LINK during egg-laying. (A) A schematic outlining when mNG::fibulin-1, hemicentin::mNG, α1-type IV collagen::mRuby2, and perlecan::mNG were imaged at the utse–seam B-LINK. (B) Quantification of mean florescence intensity of each B-LINK matrix component from mid-L4 through adult decline in egg-laying stage as a percentage of the mean fluorescence intensity of that component in the onset of egg-laying adult (n = 10 all stages). Mid-L4 through onset of egg-laying data shown previously in Fig. 4 B. Black arrows indicate fluorescence time points shown in C. (C) Fluorescence images of hemicentin::mNG, mNG::fibulin-1, α1-type IV collagen::mRuby2, and perlecan::mNG at the B-LINK at the adult peak egg-laying, adult decline in egg-laying 120 h/∼day 3 adult, and adult decline in egg-laying 168 h/∼day 4 adult stages. Colors are inverted to improve visibility. Scale bars, 10 µm.

Hemicentin and fibulin-1 decline at the utse–seam B-LINK during egg-laying. (A) A schematic outlining when mNG::fibulin-1, hemicentin::mNG, α1-type IV collagen::mRuby2, and perlecan::mNG were imaged at the utse–seam B-LINK. (B) Quantification of mean florescence intensity of each B-LINK matrix component from mid-L4 through adult decline in egg-laying stage as a percentage of the mean fluorescence intensity of that component in the onset of egg-laying adult (n = 10 all stages). Mid-L4 through onset of egg-laying data shown previously in Fig. 4 B. Black arrows indicate fluorescence time points shown in C. (C) Fluorescence images of hemicentin::mNG, mNG::fibulin-1, α1-type IV collagen::mRuby2, and perlecan::mNG at the B-LINK at the adult peak egg-laying, adult decline in egg-laying 120 h/∼day 3 adult, and adult decline in egg-laying 168 h/∼day 4 adult stages. Colors are inverted to improve visibility. Scale bars, 10 µm.

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