Figure S3.
Dependency of B-LINK matrix components on each other at the B-LINK. (A) A schematic diagram showing the timing of RNAi knockdown and analysis of utse–seam B-LINK matrix component levels. (B–D) Mean fluorescence intensity of B-LINK matrix components α1-type IV collagen::mRuby2, mNG::fibulin-1, and perlecan::mNG on control (L4440/T444T empty vectors) and corresponding B-LINK matrix component RNAi. The role of hemicentin in recruiting other B-LINK matrix components is shown in Figs. 7 and S2. lin-35(n745) was used for fibulin-1 RNAi to sensitize worms for more efficient knockdown. Box edges in boxplots depict the 25th and 75th percentiles, the line in the box indicates the median value, and whiskers mark the minimum and maximum values. ****P < 0.0001, *P < 0.05, one-way ANOVA followed by post hoc Dunnet’s test, unpaired two-tailed t test for fibulin-1 RNAi.

Dependency of B-LINK matrix components on each other at the B-LINK. (A) A schematic diagram showing the timing of RNAi knockdown and analysis of utse–seam B-LINK matrix component levels. (B–D) Mean fluorescence intensity of B-LINK matrix components α1-type IV collagen::mRuby2, mNG::fibulin-1, and perlecan::mNG on control (L4440/T444T empty vectors) and corresponding B-LINK matrix component RNAi. The role of hemicentin in recruiting other B-LINK matrix components is shown in Figs. 7 and S2. lin-35(n745) was used for fibulin-1 RNAi to sensitize worms for more efficient knockdown. Box edges in boxplots depict the 25th and 75th percentiles, the line in the box indicates the median value, and whiskers mark the minimum and maximum values. ****P < 0.0001, *P < 0.05, one-way ANOVA followed by post hoc Dunnet’s test, unpaired two-tailed t test for fibulin-1 RNAi.

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