Figure S1.
β3 integrin expression level is unchanged upon loss of β1 integrin. (A–C) Western blot of total cell lysate (A) and quantification (B and C) confirmed the deletion of β1 integrin or ICAP-1 in osteoblast cells. Tubulin is used as loading control. (E) The deletion of β1 integrin and ICAP-1 do not affect the expression of β3 integrin. Actin is used as loading control. (D) The expression of β3 integrin mRNA is not changed upon deletion of β1 integrin or ICAP-1. Luminescence signal is normalized to the β1 integrin+/+- icap-1+/+. ns, adjusted P value > 0.05; *, P value ≤ 0.05; **, P value ≤ 0.01; ***, P value ≤ 0.001; ****, P value ≤ 0.0001. (F) Intensity of ppMLC staining decorating the stress fibers, normalized to cell area. Error bars represent SD. N ≥ 211 cells. ns, adjusted P value > 0.05; *, P value ≤ 0.05; **, P value ≤ 0.01; ***, P value ≤ 0.001; ****, P value ≤ 0.0001. (G) The level of the double phosphorylation (T18/S19) of the MLC was assessed and quantified via Western blot against the total level of MLC of cell lysates of cells spread for 4 h on fibronectin-covered glass. Error bars represent SD. ns, adjusted P value > 0.05; *, P value ≤ 0.05; **, P value ≤ 0.01; ***, P value ≤ 0.001; ****, P value ≤ 0.0001. Source data are available for this figure: SourceData FS1.

β3 integrin expression level is unchanged upon loss of β1 integrin. (A–C) Western blot of total cell lysate (A) and quantification (B and C) confirmed the deletion of β1 integrin or ICAP-1 in osteoblast cells. Tubulin is used as loading control. (E) The deletion of β1 integrin and ICAP-1 do not affect the expression of β3 integrin. Actin is used as loading control. (D) The expression of β3 integrin mRNA is not changed upon deletion of β1 integrin or ICAP-1. Luminescence signal is normalized to the β1 integrin+/+- icap-1+/+. ns, adjusted P value > 0.05; *, P value ≤ 0.05; **, P value ≤ 0.01; ***, P value ≤ 0.001; ****, P value ≤ 0.0001. (F) Intensity of ppMLC staining decorating the stress fibers, normalized to cell area. Error bars represent SD. N ≥ 211 cells. ns, adjusted P value > 0.05; *, P value ≤ 0.05; **, P value ≤ 0.01; ***, P value ≤ 0.001; ****, P value ≤ 0.0001. (G) The level of the double phosphorylation (T18/S19) of the MLC was assessed and quantified via Western blot against the total level of MLC of cell lysates of cells spread for 4 h on fibronectin-covered glass. Error bars represent SD. ns, adjusted P value > 0.05; *, P value ≤ 0.05; **, P value ≤ 0.01; ***, P value ≤ 0.001; ****, P value ≤ 0.0001. Source data are available for this figure: SourceData FS1.

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